THE ACTIVE SITE OF BOVINE PANCREATIC RIBONUCLEASE: AN EXAMPLE OF SOLVENT MODULATED SPECIFICITY
- 1 July 1994
- journal article
- Published by Bentham Science Publishers Ltd. in Protein & Peptide Letters
- Vol. 1 (1) , 60-65
- https://doi.org/10.2174/0929866501666220422152935
Abstract
A dynamic role for conserved water molecules in modulating the substrate specificity of ribonuclease is proposed based upon X-ray and neutron crystallographic studies. The structures of ribonuclease complexed with a transition state and substrate analogs are compared with the high resolution structure of the unliganded enzyme. Two conserved water molecules change their donor-acceptor roles in hydrogen bonds for accommodation of either uracil or cytosine in the B1 pocket.Keywords
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