Glucose transporters are abundant in cells with "occluding" junctions at the blood-eye barriers.
- 1 June 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (11) , 4261-4264
- https://doi.org/10.1073/pnas.87.11.4261
Abstract
We studied the distribution of the "erythroid/brain" glucose transporter protein in the human and rat eye by immunocytochemistry with monoclonal and polyclonal antibodies to the C terminus of the human erythrocyte glucose transporter. We found intense immunocytochemical staining in the endothelium of microvessels of the retina, optic nerve, and iris but not in microvessels of the choroid, ciliary body, sclera, and other retro-orbital tissues. In addition, we found marked immunocytochemical staining of retinal pigment epithelium, ciliary body epithelium, and posterior epithelium of the iris. The common feature of all those endothelial and epithelial cells that stained intensely for the glucose transporter is the presence of "occluding" intercellular junctions, which constitute the anatomical bases of the blood-eye barriers. We propose that a high density of the glucose transporter is a biochemical concomitant of epithelial and endothelial cells with barrier characteristics, at least in tissues that have a high metabolic requirement for glucose.This publication has 37 references indexed in Scilit:
- Identification and characterization of the glucose transporter of the blood-brain barrier by cytochalasin B binding and immunological reactivity.Proceedings of the National Academy of Sciences, 1984
- Unidirectional transport mechanism of horseradish peroxidase in the vessels of the iris.1984
- Use of anti-basement membrane antibodies to distinguish blood vessel capillaries from lymphatic capillariesThe American Journal of Surgical Pathology, 1983
- The structural basis of the blood-ocular barriersExperimental Eye Research, 1977
- Ocular vascular and epithelial barriers to microperoxidase.1975
- Ultrastructural studies of the blood-aqueous barrier. I. Transport of an electron-dense tracer in the iris and ciliary body of the mouse.1971
- An electron microscopic study of the permeability of iris capillaries to horseradish peroxidase in the vervet monkey (Cercopithecus aethiops)Cell and tissue research, 1971
- JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN THE VERTEBRATE BRAINThe Journal of cell biology, 1969
- Studies on the permeability of the blood-retinal barrierExperimental Eye Research, 1966
- Facilitated transfer of glucose from blood into brain tissue.The Journal of Physiology, 1965