Metabolism of 15NH3 in Organotypic Cerebellar Explants and Cultured Astrocytes: Studies with Gas Chromatography‐Mass Spectrometry

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Abstract
Gas chromatography‐mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady‐state level of 15NH3 was present by 1 min in both systems. Steady‐state labeling in l‐[amide‐15N] glutamine, l‐[15N]alanine, l‐[15N]glutamate, and l‐[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.