The Influence of Extracellular Hydrogen on the Metabolism of Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium

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Abstract
Strains of 3 anaerobic rumen bacteria, B. ruminicola, A. lipolytica and S. ruminantium, were able to use extracellular H2 to reduce fumarate to succinate. Each bacterium possessed membrane-bound hydrogenase and fumarate reductase activity. Membrane-bound cytochrome b was reducible by H2 and oxidizable by fumarate in each bacterium. The apparent Km values for hydrogen of the hydrogenase were 4.5 .times. 10-6 M, 1.4 .times. 10-5 M and 4.4 .times. 10-5 M for B. ruminicola, A. lipolytica and S. ruminantium, respectively. The apparent Km values for fumarate of the fumarate reductases were approximately 1.0 .times. 10-4 M for each bacterium.
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