The Cell Cycle in the Fission Yeast, Schizosaccharomyces pombe. IV. Relationship between Cell Size and Cycle Time of Non-Growing Cells Due to a Nutritional Shift-Down

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Abstract
To prepare an oversized and synchronized cell sample, exponentially growing cells of S. pombe were exposed to 8 mM hydroxyurea for 3 h, then transferred to a poor medium (0.5% malt medium on N-free minimal medium). The transferred cells were grown in a small chamber without shaking and observed by time-lapse photomicrography. Cells cultivated in the poor medium divided twice synchronously, but hardly elongated in cell length between the 2 divisions. Under this condition, the relationship between the cell size and cycle duration (the time interval between the divisions) was investigated. Cells smaller than a particular size had a negative correlation between cell size at birth and cycle duration. Cells above the particular cell size had a constant minimum cycle duration. Cells divided regardless of size at division. Cycle duration may depend on cell size at birth, not at division.

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