Determination of Phenytoin in Serum in the Presence of Barbiturates, Sulthiame and Ethosuximid by Thin-Layer Chromatography
- 1 January 1969
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 23 (2) , 175-180
- https://doi.org/10.3109/00365516909077022
Abstract
Phenytoin was determined quantitatively and specifically by a method suitable for routine use. Extracts of 1 ml serum were applied to silica gel plates with a dispensing syringe. After development, the plates were dipped into staining reagents and faded by high humidity and heat. The uniformity of the plates ensures well defined spots with constant RF values. In routine conditions, 0.5–40μg pnenytoin could be quantified corresponding to serum concentrations in the range 0–20 m/gl, the S. D. being 1 mg/l. The determination could be completed in 2 1/2 hours.Keywords
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