Doxorubicin retention and chemoresistance in human mesothelioma cell lines

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Abstract
Eight cell lines were established from the pleural effusion of 4 patients with malignant mesothelioma. The most sensitive (FCCMES‐4) and the most resistant (FCCMES‐2) mesothelioma cell lines had IC50 of 0.66 and 1.85 μM for doxorubicin in clonogenic assays, respectively. In comparison with murine leukemic P388 cells, mesothelioma cell lines were 7.5‐ to 21 ‐fold more resistant to doxorubicin. Co‐incubation with verapamil significantly increased doxorubicin retention in one of the cell lines (FCCMES‐2) expressing P‐glycoprotein in 16.8% of the cells. These results indicate that doxorubicin resistance may be intrinsic in refractory mesothelioma patients and P‐glycoprotein‐mediated drug efflux may be involved in resistance of some of the mesotheliomas.
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