Response of Cultured IEC-17 Normal Rat Intestinal Epithelial Cells to X Radiation

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Abstract
The growth parameters and radiosensitivity of normal rat intestinal epithelial cells, IEC-17, were studied. The cells were cultured by standard methods and exposed to an array of doses (1-12 Gy [grays]) of 250 kVp X-rays. The survival curves generated exhibited no initial shoulder and were bimodal. The D0 [dose required to reduce survival to 0%] of the 1st component was .apprx. 0.2 Gy and the 2nd component, 5.0 Gy. The ability of this cell line to repair sublethal lesions was examined by fractionation studies; repair was completed within 60 min after the 1st dose. When Chinese hamster ovary (CHO) cells were grown under the same conditions used for the IEC-17 cells and then irradiated with single doses, a typical survival curve with a D0 of 1.4 Gy was obtained. The survival curves obtained for the IEC-17 cell line are consistent with the response of a morphologically distinct single population containing 2 functionally separate types of cells.