Effect of Heat-Treatment on the Respiration of Trypanosoma Cruzi Used for the Cultivation of Endamoeba Histolytica

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Abstract
Summary 1. E. histolytica heat-treated at 45°C., 48°C., and 50°C. for 10 minutes survived when transplanted onto unheated T. cruzi or trypanosomes heated at 45°C. and 48°C. for 10 minutes, but failed to survive when associated with trypanosomes heat-treated at 50°C. 2. Subcultures of T. cruzi after heating at 48°C. and 50°C. failed to produce growth. 3. Oxygen uptake studies indicated that there is a pronounced respiration with 1 ml. of T. cruzi with a count of 100,000,000 per ml. heat-treated at 48°C. for 10 minutes, but no respiration occurs with T. cruzi heat-treated at 50°C. for 10 minutes. 4. The efficacy of T. cruzi associates in the cultivation of E. histolytica in vitro was demonstrated after heat-treatment that rendered the trypanosomes non-viable but did not extend to temperatures that inhibited their respiration.