The fluorescence spectrum and the fluorescence steady-state anisotropy of C60 in a toluene–ethanol 10 : 1 (v/v) mixture have been obtained at room temperature and at 140 K. At 140 K, where molecular rotation is negligible during the excited-state lifetime, essentially zero fluorescence anisotropy was measured, irrespective of the excitation wavelength. It is concluded, in accordance with theoretical expectations, that the fluorescence of C60 is intrinsically unpolarized. C60 is the first organic molecule to show this behaviour.