Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.
Open Access
- 1 October 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 35 (10) , 2127-2129
- https://doi.org/10.1093/clinchem/35.10.2127
Abstract
This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.This publication has 6 references indexed in Scilit:
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