PHOSPHOLIPID METABOLISM IN RAT LIVER SLICES: EFFECT OF HYPOPHYSECTOMY AND ADRENALECTOMY ON THE LABELLING OF PHOSPHOLIPIDS WITH ACETATE-1-C14

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Abstract
In rats fed ad libitum the labelling of the acetone-insoluble lipids (phospholipids) of liver slices respiring in the presence of acetate-1-C14 was decreased 2 days after the removal of either the pituitary or the adrenal glands. The effect was not observed 4 days after hypophysectomy. Moreover, the effect was not observed with liver slices from hypophysectomized or adrenalectomized animals that were either deprived of food for the 12 hours immediately prior to killing, or force-fed with glucose 4 hours before killing. Hypophysectomy caused a decrease in the labelling of the fatty acids in the acetone-soluble fraction remaining in solution after precipitation of the phospholipids. This decrease was observed both in liver slices from animals fed ad libitum and in those from fasted (12 hours) animals. In rats fed ad libitum the labelling of the non-esterified cholesterol of the liver slices was decreased by hypophysectomy, but not by adrenalectomy. It is concluded that the decrease in the labelling of the acetone-insoluble lipids in liver slices from operated rats was primarily the result of a change in the nutritional status of the operated animals. The data are insufficient to permit any conclusions concerning the cause of the decrease in labelling of either the fatty acids of the acetone-soluble lipids, or the non-esterified cholesterol.

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