EFFECT OF LIPID PEROXIDATION ON p‐AMINOHIPPURATE TRANSPORT BY RAT KIDNEY CORTICAL SLICES

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Abstract
1 The effect of lipid peroxidation on p-aminohippurate transport by rat kidney slices was examined. 2 Ascorbic acid and Fe2+ promoted lipid peroxidation of rat renal cortical slices in a dose-related manner. 3 Ascorbic acid (1.0 mM) and Fe2+ (0.4 mM) increased tissue water and decreased the accumulation of p-aminohippurate. 4 The addition of N,N'-diphenyl-p-phenylenediamine (antioxidant), at a concentration of 1 × 10−6m, completely inhibited the peroxidation and recovered the accumulation of p-aminohippur ate. 5 The apparent Km of p-aminohippurate uptake was increased by ascorbic acid and Fe2+ with no change in the apparent V. 6 These data suggest that ascorbic acid and Fe2+ can cause a significant alteration in p-aminohippurate and water transport of renal cortical slices and that these effects can be correlated with lipid peroxidation.