Expression of Virus-specific, Thymus-specific and Tumour-specific Antigens in Lymphoblastoid Cell Lines Derived from Marek's Disease Lymphomas

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Abstract
The expression of virus, thymus [T] and tumor[Tu]-specific antigens was studied in 2 Marek''s disease [chicken] lymphoblastoid cell lines (MSB-1 and HPRS-line 2). The proportions of cells which spontaneously expressed virus antigens or which formed infective centers in vitro were considerably higher in MSB-1 than in HPRS-line 2, but did not exceed 10%. In contrast to virus antigens, T-specific and Tu-specific antigens were expressed on the majority of the cells. Treatment with IdUrd [iododeoxyuridine] increased the proportion of cells forming infective centers in both cell lines and the proportion of cells forming infective centers in IdUrd-treated and in untreated cultures was noted during continuous subculture of both cell lines. Direct evidence for the presence of virus-specific antigens in MSB-1 cells was obtained by immunodiffusion. Lymphoblastoid cells are apparently unable to synthesize a major precipitating glycoprotein antigen (A antigen) normally associated with infection of permissive cells with MDV [Marek''s disease virus]. Analysis of surface proteins of normal T cells labeled by lactoperoxidase-catalyzed iodination showed that T-specific determinants are associated with iodinated polypeptides in the MW ranges 45,000-47,000, 50,000-58,000 and 90,000-150,000. The major T-specific polypeptide exposed at the surface of normal T cells was in the MW range of 50,000-58,000. Surface proteins of lymphoblastoid cells were not accessible to iodination.

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