Determination of the packaging capacity of bacteriophage VWB
Open Access
- 1 February 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (2) , 1129-1132
- https://doi.org/10.1128/jb.172.2.1129-1132.1990
Abstract
VWB is a temperate bacteriophage whose chromosome has cohesive ends. VWB can stably package modified chromosomes that contain insertions of up to about 4 kilobases of foreign DNA. Phage particles containing extra DNA differ from the wild type in their increased sensitivity to chelating agents. Because of these properties, VWB is a promising cloning vector for Streptomyces venezuelae.This publication has 18 references indexed in Scilit:
- Plasmid pIJ699, a multi-copy positive-selection vector for StreptomycesGene, 1988
- [17] A sytematic approach to chemical DNA sequencing by subcloning in pGV451 and derived vectorsPublished by Elsevier ,1987
- Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicatorMolecular Genetics and Genomics, 1986
- A restriction endonuclease map of Streptomyces phage VWBMolecular Genetics and Genomics, 1985
- Morphological and Molecular Characterization of Several Actinophages Isolated from Soil Which Lyse Streptomyces cattleya or S. venezuelaeMicrobiology, 1984
- Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coliPlasmid, 1984
- Cloning and Expression of the Tyrosinase Gene from Streptomyces antibioticus in Streptomyces lividansMicrobiology, 1983
- Dispensable sequences and packaging constraints of DNA from the Streptomyces temperate phage φC31Gene, 1981
- Packaging of the bacteriophage λ chromosome: Effect of chromosome lengthVirology, 1977
- Deletion mutants of bacteriophage lambdaJournal of Molecular Biology, 1971