A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates
- 1 December 1995
- journal article
- research article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 4 (3) , 259-268
- https://doi.org/10.1007/bf02779019
Abstract
A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.Keywords
This publication has 29 references indexed in Scilit:
- Rapid Reaction Analysis of the Catalytic Cycle of the EcoRV Restriction EndonucleaseBiochemistry, 1995
- Vaccinia DNA topoisomerase I: Single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactionsBiochemistry, 1994
- EcoRV restriction endonuclease: communication between catalytic metal ions and DNA recognitionBiochemistry, 1992
- Discrimination between DNA sequences by the EcoRV restriction endonucleaseBiochemistry, 1989
- Modes of DNA cleavage by the EcoRV restriction endonucleaseBiochemistry, 1988
- Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitroBiochemistry, 1987
- Trans-complementable copy-number mutants of plasmid ColE1Nature, 1980
- Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAsBiochemistry, 1977
- Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrateJournal of Molecular Biology, 1975
- The interaction of closed circular DNA with intercalative dyesJournal of Molecular Biology, 1968