Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine
- 3 December 1981
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 214 (4525) , 1125-1129
- https://doi.org/10.1126/science.6272395
Abstract
A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.This publication has 32 references indexed in Scilit:
- Foot-and-mouth disease virus: Immunogenicity and structure of fragments derived from capsid protein VP3 and of virus containing cleaved VP3Veterinary Microbiology, 1982
- Molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genesNature, 1981
- Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coliNature, 1981
- Direct expression in Escherichia coli of a DNA sequence coding for human growth hormoneNature, 1979
- Foot-and-Mouth Disease Virus Immunogenic Capsid Protein VPT: N-Terminal Sequences and Immunogenic Peptides Obtained by CNBr and Tryptic CleavagesIntervirology, 1978
- Biochemical Mapping of the Foot-and-Mouth Disease Virus GenomeJournal of General Virology, 1977
- Foot-And-Mouth DiseaseAnnual Review of Microbiology, 1968
- Foot-and-mouth disease in swineArchiv für die gesamte Virusforschung, 1967
- A third antigenic component associated with foot-and-mouth disease infectionVirology, 1966
- A Comparison of different routes of inoculation of cattle for detection of the virus of foot-and-mouth diseaseEpidemiology and Infection, 1952