ESTABLISHMENT OF A CELL LINE FROM EMBRYONIC TISSUES OF THE FLESHFLY, SARCOPHAGA PEREGRINA (INSECTA: DIPTERA)

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Abstract
A continuous cell line was established from embryonic tissues of the fleshfly, S. peregrina, and was designated as NIH-SaPe-4. The primary culture was initiated in Oct., 1977, and the cell line was passed 68 times during the following year. The cells were heterogeneous in morphology. Most cells were diploid and their chromosomes consisted of 4 metacentric, 6 submetacentric and 2 micro chromosomes. The population doubling time of the cell line was about 30 h. The cells grew faster in Mitsuhasi-Maramorosch''s medium than in Schneider''s medium. The cells were either stored in the usual medium at 5.degree. C for about 3 mo. or in a medium containing 10% glycerol at -80.degree. C for a longer period. Cell growth was suppressed by 20-hydroxyecdysone when at a greater concentration than 0.01 .mu.g/ml; insulin showed no effects on cell growth at a strength of 0.4 and 0.04 IU/ml.