A Simple Method for Determination of Desoxypentose Nucleic Acid in Tissue Cultures
- 1 July 1957
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 95 (3) , 458-462
- https://doi.org/10.3181/00379727-95-23251
Abstract
A new method for the quantitative determination of DNA in tissue cells is presented. It involves dissolving the cells in NaOH and heating to hydrolyze RNA; precipitating DNA with salmine; extracting DNA from the precipitate with hot acid salt solution; and measuring the amount of DNA by means of light absorbence at 268 m[mu]. Recovery of added DNA from cell extracts and in the presence of mononucleotides is nearly 100%. Absorption spectrum studies indicate a high degree of specificity. With microtechnique the determination of less than 1 [mu]g of DNA is feasible.Keywords
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