Serological Studies of Plant Viruses

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Abstract
The viruses of tobacco mosaic, spot necrosis, ring spot, and cucumber mosaic were studied by serological technique. Several methods of purification were used in an attempt to free them from contaminating antigenic substances. Rabbits were immunized against these various purified preparations and also against crude extracts of diseased and healthy tobacco plants. The results of cross precipl-tin tests and precipltin adsorption tests indicate (1) that viruses may be freed from antigenic constituents of healthy plants by several methods of purification; (2) that the juice from virus-diseased plants contains, in addition to the antigenic constituents of normal plants, an antigenic fraction which, by the methods employed, was inseparable from the virus; (3) that this antigenic fraction appeared to be specific for a particular virus. The serological technique appears to be of value in the study of plant viruses.

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