AFFINITY CHROMATOGRAPHY OF ALPHA-AMYLASES ON CROSS-LINKED STARCH

  • 1 January 1976
    • journal article
    • research article
    • Vol. 58  (11-1) , 1299-1302
Abstract
Affinity chromatography on cross-linked starch affords a simple and rapid procedure for .alpha.-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the .alpha.-amylase which is then eluted with 2 M maltose solution. .alpha.-Amylase can be obtained in a pure form with a 60% yield. The exoenzyme .beta.-amylase (EC 3.2.1.2) is not retained by the support and is eluted with other contaminant proteins. This procedure allows the separation of the endo- and exoamylase activities.