Simple determination of the coccidiostat robenidine in poultry feed

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Abstract
1,3-Bis(4-chlorobenzylideneamino)guanidinium chloride (robenidine) is a new preventive coccidiostat used in poultry feed. A fast, accurate method of determination is proposed, which consists in extraction of the robenidine from the feed, purification of the extract by means of thin-layer chromatography and development of a yellow coloration by addition of sodium hydroxide to a solution of the robenidine in dimethylformamide. The absorbance is measured at 464 nm and is proportional to the concentration of robenidine between 0 and 3 µg ml–1. No interference is observed during its determination in fatty or pigmented feeds.

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