Enzymic desulphation of porphyran
- 1 September 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 80 (3) , 449-453
- https://doi.org/10.1042/bj0800449
Abstract
The enzyme responsible for the desulphation of porphyran by extracts of Porphyra umbilicalis has been purified 22-fold by adsorption on calcium phosphate gel followed by elution with sodium acetate. A turbidimetric assay method has been developed. The effect on the enzyme of pH and of various activators and inhibitors has been studied. It is dependent on the presence of a bi- or ter-valent cation, which is not Mg2+, and is markedly activated by borate.Keywords
This publication has 3 references indexed in Scilit:
- Carbohydrase and sulphatase activities of Porphyra umbilicalisBiochemical Journal, 1961
- Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate estersBiochemical Journal, 1961
- Studies on sulphatases. 5. The determination of inorganic sulphate in the study of sulphatasesBiochemical Journal, 1953