Mutant of Chinese hamster ovary cells with altered mannose 6-phosphate receptor activity is unable to synthesize mannosylphosphoryldolichol.
- 1 April 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (7) , 2296-2300
- https://doi.org/10.1073/pnas.79.7.2296
Abstract
B4-2-1 is a Chinese hamster ovary cell mutant previously isolated and characterized as deficient in mannose 6-phosphate receptor activity. B4-2-1 is a pleiotropic mutant, defective in biosynthesis of asparagine-linked oligosaccharides. B4-2-1 is unable to synthesize mannosylphosphoryldolichol; the consequences of this defect on glycosylation are biosynthesis of 1 major lipid-linked oligosaccharide, characterized by its resistance to endoglycosidase H and decreased size; this oligosaccharide is similar to a minor species of lipid-linked oligosaccharide found in parental cells; transfer of this oligosaccharide to newly synthesized proteins; and absence of normal high-mannose oligosaccharides on mature glycoproteins isolated from B4-2-1; glycoproteins from the mutant contain complex oligosaccharides as well as endoglycosidase H-resistant, .alpha.-mannosidase-sensitive species. While the glycosylation defect may alter adversely the function of several glycoproteins in the mutant, including that of the mannose 6-phosphate receptor, it appears to have no effect on the formation or function of the mannose 6-phosphate recognition marker on acid hydrolases of B4-2-1.Keywords
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