Intracellular proteinase ofLactococcus lactissubsp.lactisNCDO 763
- 1 May 1989
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Dairy Research
- Vol. 56 (5) , 765-778
- https://doi.org/10.1017/s0022029900029344
Abstract
Summary: An intracellular proteinase was purified fromLactococcus lactissubsp.lactisNCDO 763 after spheroplast formation from cell wall proteinase-deficient variants. The proteinase was active at pH 7·5 and 45 °C and affected by metalloenzyme inhibitors. Its specificity, determined on B-chain of insulin, was thermolysin-like. The B-chain of insulin was hydrolysed rapidly while hydrolysis of β-casein was slower. This enzyme has aMrof ∽ 93000.This publication has 31 references indexed in Scilit:
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