Increased purine nucleotide binding, altered polypeptide composition, and thermogenesis in brown adipose tissue mitochondria of cold-acclimated rats

Abstract
Rapid increases in atractyloside-insensitive binding of purine nucleotides (ADP or GDP) and in a polypeptide of MW 32,000 occur in brown adipose tissue mitochondria of the rat during acclimation to cold. The increased binding is apparent within 1 h and reaches a maximum after 3-7 days of exposure to 4.degree. C. The increase in the MW 32,000 peptide occurs more slowly and reaches a maximum after 2-3 wk. There is a simultaneous decrease in a polypeptide of MW 96,000, apparent after 1 day and reaching a maximum after 1-2. The appearance of an increased amount of the purine nucleotide-sensitive proton conductance pathway was discussed in association with the development of an enhanced thermogenic capacity of brown adipose tissue mitochondria during acclimation of the rat to cold.

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