Two‐dimensional impedance studies of BSA buoyant density separated human erythrocytes
Open Access
- 1 January 1985
- Vol. 6 (1) , 13-21
- https://doi.org/10.1002/cyto.990060104
Abstract
Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyant density but did show a shift with opacity, radio frequency impedance divided by dc impedance. This new electronic parameter of opacity can be related to cell age, since both it and cell age are directly related to buoyant density. This increase in opacity with buoyant density is correlated with a change in shape.Keywords
This publication has 15 references indexed in Scilit:
- What is red cell deformability?Scandinavian Journal of Clinical and Laboratory Investigation, 1981
- Application of the Electrical Sizing Principle of Coulter to a New Multiparameter SystemIEEE Transactions on Biomedical Engineering, 1980
- Some observations on the dielectric properties of hemoglobin's suspending medium inside human erythrocytesBiophysical Journal, 1980
- Human erythrocyte fractionation in “percoll” density gradientsClinica Chimica Acta; International Journal of Clinical Chemistry, 1979
- Buoyant Density Separation with Linear Gradients of Bovine Serum Albumin and Analysis by Centrifugal Cytology and Flow TechniquesPublished by Springer Nature ,1979
- Flow-system measurement of cell impedance properties.Journal of Histochemistry & Cytochemistry, 1979
- Biochemical characterization of density-separated human erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Buoyant density separation of cells. I. The buoyant distribution of guinea pig bone marrow cells.Journal of Histochemistry & Cytochemistry, 1975
- Density gradient systemAnalytical Biochemistry, 1972
- THE DISTRIBUTION OF BUOYANT DENSITY OF HUMAN ERYTHROCYTES IN BOVINE ALBUMIN SOLUTIONSProceedings of the National Academy of Sciences, 1964