Enzymic hydrolysis of amino acid derivatives of benzocaine

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Abstract
1. Enzymic hydrolysis of amino acid derivatives of benzocaine occurs with homo-genates of rat muscle, kidney, liver and other tissues, and in human blood serum. 2. Marked differences in rates of hydrolysis depend on the type of tissue and the nature of the amino acid moiety. The highest rate of hydrolysis was observed with kidney and with DL-leucylbenzocaine as substrate; N, N-dimethylglycylbenzocaine was the poorest substrate. 3. Enzymic hydrolysis of amino acid derivatives of benzocaine was catalysed by leucylaminopeptidase, ‘pronase', ‘peptidase’ and by proteolytic enzymes in duodenal juice. Purified preparations of pepsin, trypsin and chymotrypsin do not catalyse hydrolysis.