N‐Methyl‐D‐Aspartate Receptor Activation and Ca2+ Account for Poor Pyramidal Cell Structure in Hippocampal Slices
- 1 August 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 55 (2) , 473-483
- https://doi.org/10.1111/j.1471-4159.1990.tb04160.x
Abstract
The CA1 pyramidal cells appear damaged in micrographs of guinea pig hippocampal slices incubated in normal physiological buffer at 36-37.degree. C. This is remedied if slices are incubated in modified buffers for the first 45 min. Cell morphology is improved if this buffer is devoid of added Ca2+ and much improved if it contains N-methyl-D-aspartate (NMDA) receptor antagonists or 0 mM Ca2+ and 10 mM Mg2+. The cells then appear similar to CA1 pyramidal cells in situ. These findings support the notion that NMDA receptor activation and Ca2+, acting in the period immediately after slice preparation, permanently damage CA1 pyramidal cells in vitro.Keywords
This publication has 45 references indexed in Scilit:
- The N-methyl-d-aspartate antagonists aminophosphonovaleric acid and MK-801 reduce anoxic damage to dentate granule and CA1 pyramidal cells in the rat hippocampal sliceExperimental Neurology, 1989
- Ketamine Effects on Local Cerebral Blood Flow and Metabolism in the RatJournal of Cerebral Blood Flow & Metabolism, 1987
- Properties of the interaction between ketamine and opiate binding sites in vivo and in vitroNeuropharmacology, 1987
- Elevation of the Extracellular Concentrations of Glutamate and Aspartate in Rat Hippocampus During Transient Cerebral Ischemia Monitored by Intracerebral MicrodialysisJournal of Neurochemistry, 1984
- Metabolic Stability of Hippocampal Slice Preparations During Prolonged IncubationJournal of Neurochemistry, 1984
- Microinjection of Ca++-calmodulin causes a localized depolymerization of microtubules.The Journal of cell biology, 1983
- The role of calcium in cell deathLife Sciences, 1981
- Phosphonate analogues of carboxylic acids as aminoacid antagonists on rat cortical neuronesNeuroscience Letters, 1981
- MEASUREMENTS OF RATES OF PROTEIN SYNTHESIS IN RAT BRAIN SLICESJournal of Neurochemistry, 1974
- Embedding in Epoxy Resins for Ultrathin Sectioning in Electron MicroscopyStain Technology, 1960