Interaction of 3',4'-dideoxykanamycin B with a lysosomal enzyme, N-acetyl-.BETA.-D-glucosaminidase.
- 1 January 1983
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 31 (1) , 321-324
- https://doi.org/10.1248/cpb.31.321
Abstract
The binding of 3'',4''-dideoxykanamycin B with a lysosomal enzyme, N-acetyl-.beta.-D-glucosaminidase, obtained from rat kidney was examined by affinity chromatography of the enzyme on 3'',4''-dideoxykanamycin B-conjugated Sepharose 4B. N-Acetyl-.beta.-D-glucosaminidase could be adsorbed on the affinity column. The enzyme activity was significantly increased by the addition of 3'',4''-dideoxykanamycin B. These findings indicate that 3'',4''-dideoxykanamycin B does interact with N-acetyl-.beta.-D-glucosaminidase. [This antibiotic has been shown to be nephrotoxic.].This publication has 9 references indexed in Scilit:
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