Molecular cloning of hepatic 5‐aminolevulinate synthase
- 1 October 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 144 (1) , 95-99
- https://doi.org/10.1111/j.1432-1033.1984.tb08435.x
Abstract
Hepatic 5-aminolevulinate synthase was induced in chick embryos by administration of the porphyrinogenic drugs 2-allyl-2-isopropylacetamide and 3,5-diethoxycarbonyl-1,4-dihydrocollidine. A cDNA [complementary DNA] library was constructed from drug-induced liver mRNA and clones containing sequences coding for 5-aminolevulinate synthase were identified by hybrid-selected translation. The identity of these clones was confirmed by comparing DNA sequence data with the amino acid sequence of peptides from purified 5-aminolevulinate synthase. From Northern blot analysis the size of the mRNA for 5-aminolevulinate synthase was estimated to be 2800 base pairs, .apprx. 600 base pairs more than that required to code for the primary translation product of relative molecular mass 74,000.This publication has 17 references indexed in Scilit:
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