Axonal Transport of the Ca2+‐Dependent Protein Modulator of 3′:5′‐Cyclic‐AMP Phosphodiesterase in the Rabbit Visual System
- 1 July 1980
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 35 (1) , 242-248
- https://doi.org/10.1111/j.1471-4159.1980.tb12510.x
Abstract
Water-soluble proteins were extracted from individual retinas, optic nerves, combined optic tracts and lateral geniculate bodies and superior colliculi of rabbits at 1, 3 and 18 days after injection of [3H]leucine into the right eye. The Ca2+-dependent protein modulator of 3'':5''-cyclic-AMP phosphodiesterase (calmodulin) was isolated from these samples by a 2-step polyacrylamide gel electrophoresis procedure. An analysis of the radioactivity incorporated into the total soluble proteins and the calmodulin revealed that most of the calmodulin was axonally transported at a slow rate (2-4 mm/day) and represented about 0.45% of the total transported soluble protein.Keywords
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