COMPARATIVE EFFICIENCY OF 3 DIFFERENT TESTS FOR ESTIMATION OF MUTAGENICITY OF SOME FACTORS IN MAMMALS .2. THE FREQUENCY OF ABNORMAL SPERM HEAD IN MICE TREATED WITH DIFFERENT FACTORS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 16  (8) , 1397-1403
Abstract
The effect of a number of chemical and physical agents (cyclophosphamide (CTX)-50, 100 and 300 mg/kg; N-nitroso-N-methylurea (NMU)-50 mg/kg; N-nitrosomorpholine (NM)-50 and 150 mg/kg; CdCl2-1, 2 and 4 mg/kg; ZnCl2-15 mg/kg; microwaves and .gamma.-rays 60Co on the frequency of abnormal sperm heads (ASH) in mouse Fi hybrids (C57BL .times. CBA)) was studied. The agents possessing mutagenicity in the dominant lethal mutations (DLM) and bone marrow chromosome aberrations assay (CTX, NMU and .gamma.-rays) significantly increased the ASH frequency within 35 days after treatment at maximum doses. CdCl2 induced no mutagenic effect in the DLM test and increased the ASH frequency. The increase in the ASH frequency in all cases are accompanied by a reduction of the testicular weight and an increase in preimplantation losses and a reduction of the level of efficient matings when premeiotic germ cells were treated. The occurrence of ASH may be due not only to the mutagenic but also to cytolytic or/and cytotoxic effects of the compounds. The ASH test fan be used for mutagenicity prescreening of different factors provided their doses are high enough.

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