A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.
Open Access
- 1 November 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 2 (2) , 144-148
- https://doi.org/10.1101/gr.2.2.144
Abstract
We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.Keywords
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