Studies on the Growth in Culture of Plant Cells

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Abstract
The influence of shaking rates (expressed as revolutions per min) on orbital shaking platforms (1 in (2.54 cm) diam. rotary motion) on the growth of cell suspension cultures of Acer pseudoplatanus L. and Atropa belladonna cultivar lutea Döll are described. By following cell growth and respiration and the levels of oxygen and carbon dioxide in the media during the progress of incubation it is concluded that the reduction of growth at sub-optimal shaking rates is not due to oxygen deficiency or toxic accumulation of carbon dioxide. The growth of the Atropa cell suspension in ‘closed systems’ has been studied by the development of modified culture vessels and evidence obtained that the reduced growth in the systems is due to the formation by the cultures of an unidentified volatile growth inhibitor and not to either oxygen depletion or toxic accumulation of either carbon dioxide or ethylene. It is suggested that the reduced growth in ‘open systems’ cultures at sub-optimal shaking speeds is either due to retention of this volatile inhibitor or to restriction of nutrient uptake by the existence of a stationary liquid-phase boundary to the cells.

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