Abstract
Numerical analysis of the relationships among rapidly growing, scotochromogenic mycobacteria was carried out; a dendrogram was prepared by a single linkage method and the separation between species was tested. The following were distinct species: Mycobacterium aichiense, M. rhodesiae, M. chubuense, M. obuense, M. gilvum, M. duvalii, M. flavescens and M. vaccae. M. tokaiense was reduced to a synonym of M. chubuense. M. parafortuitum, M. aurum and M. neoaurum were considered subspecies of M. parafortuitum or part of a M. parafortuitum complex. The separation between species was tested by comparing the extent of overlap of 1 sp. with that of another. When 2 spp. showed no such overlap, they were regarded as distinct. The results of the test of separation agreed well with taxonomic relationships shown by the dendrogram. Characters useful for differentiating the species were found.

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