Production and evaluation of monospecific antibodies for a processing-independent sequence of human progastrin
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 49 (2) , 173-182
- https://doi.org/10.3109/00365518909105418
Abstract
We have produced a series of monospecific antibodies in five rabbits by immunization with a peptide corresponding to the preprogastrin sequence 76–88. Both antibody titres (0.2 to 3.0x106), indexes of heterogeneity (−1.0), and binding affinities (K°eff-0.2 to 3.7X1012 lxmol−1) were very high. The specificity for the N-terminus of the progastrin fragment was unique because even a conservative amino acid substitution five positions from the centre of binding diminished the binding by 90% for all antibodies. We conclude that these monospecific antibodies are well suited for development of a processing-independent analysis of progastrin and its products.Keywords
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