Abstract
This paper presents the results of experiments on the growth and differentiation of embryos of three species of Sargassum (Phaeophyta, Sargassaceae) from Hawaii. The species studied were S. echinocarpum J. Agardh, S. obtusifolium J. Agardh, and S. polyphyllum J. Agardh. The embryos were grown for 20 days, each set at five salinities and four temperatures in seawater from four different sources. Maximum elongation occurred most frequently at 24°C and at 30–35‰ salt. The interaction of these factors, and the relationship of the results to conditions in the intertidal zone and phenology of the species, is discussed. Elongation and expansion of leaf initials occurred in three of four types of seawater; the expansion of leaf initials failing to occur in the Eniwetok (an atoll) medium. The results are discussed in relation to the absence of Sargassum species from most atolls.

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