Analysis of urinary nucleosides. II. Comparison of mass spectrometric methods for the analysis of urinary nucleosides
- 16 August 2001
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 15 (18) , 1701-1707
- https://doi.org/10.1002/rcm.428
Abstract
Qualitative and quantitative analyses of urinary nucleosides have diagnostic potential as tumour markers. We have developed separation techniques linked to mass spectrometric detection in order to overcome the problems associated with past identification and quantitation methods. The three methods of analysis utilised were: gas chromatography/mass spectrometry (GC/MS), high‐performance liquid chromatography/ion‐trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadrupole mass spectrometry (CapLC/TQMS). Here we compare the relative effectiveness of each of the techniques for subsequent application in the systematic study of urinary nucleoside profiles in cancer patients. All three methods proved to be valuable techniques for such urinary nucleoside analyses, and a combination rather than one single choice is concluded as the ideal. Copyright © 2001 John Wiley & Sons, Ltd.Keywords
This publication has 16 references indexed in Scilit:
- Comparison between capillary and nano liquid chromatography–electrospray mass spectrometry for the analysis of minor DNA–melphalan adductsJournal of Chromatography B: Biomedical Sciences and Applications, 2000
- Development of a Purification Procedure for the Isolation of Nucleosides from Urine Prior to Mass Spectrometric AnalysisNucleosides, Nucleotides and Nucleic Acids, 2000
- Breast cancer prognostic significance of some modified urinary nucleosidesCancer Letters, 1996
- Phenylglycidyl ether adducts of 2′-deoxycytidine and 2′-deoxyadenosine: Stability in solution and structure analysis by electrospray tandem mass spectrometryJournal of the American Society for Mass Spectrometry, 1996
- Gas chromatography/mass spectrometric analysis of urinary nucleosides in cancer patients; potential of modified nucleosides as tumour markersRapid Communications in Mass Spectrometry, 1993
- Chapter 3 Ribonucleosides in Body Fluids: On-Line Chromato-Graphic Cleanup and Analysis by A Column Switching TechniquePublished by Elsevier ,1990
- Determination of pseudouridine at submicromolar concentrations by cathodic stripping voltammetry at a mercury electrodeAnalytica Chimica Acta, 1985
- Analysis of Pseudouridine by Fluorescence SpectrometryAnalytical Letters, 1985
- tRNA breakdown products as markers for cancerCancer, 1979
- Quantitative high-performance liquid chromatography of nucleosides in biological materialsJournal of Chromatography A, 1978