Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis
- 1 August 1994
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 176 (16) , 5177-5180
- https://doi.org/10.1128/jb.176.16.5177-5180.1994
Abstract
Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene constructed in the B. subtilis chromosome. The phosphorylated form of the DegU protein seems to be a regulator for expression of the sigD gene.Keywords
This publication has 28 references indexed in Scilit:
- Regulatory Proteins That Control Late-Growth DevelopmentPublished by American Society for Microbiology ,2014
- SinI modulates the activity of SinR, a developmental switch protein of Bacillus subtilis, by protein-protein interaction.Genes & Development, 1993
- Sequencing and analysis of the Bacillus subtilis lytRABC divergon: A regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifierJournal of General Microbiology, 1992
- Molecular cloning and sequencing of the upstream region of the major Bacillus subtilis autolysin gene: a modifier protein exhibiting sequence homology to the major autolysin and the spoIID productJournal of General Microbiology, 1992
- Deduced polypeptides encoded by the Bacillus subtilis sacU locus share homology with two-component sensor-regulator systemsJournal of Bacteriology, 1988
- Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systemsJournal of Bacteriology, 1988
- Molecular Cloning of a Gene Affecting the Autolysin Level and Flagellation in Bacillus subtilisMicrobiology, 1988
- Characterization and mapping of the Bacillus subtilis prtR geneJournal of Bacteriology, 1987
- Purification and Properties of Autolytic Endo- -N-acetylglucosaminidase and the N-Acetylmuramyl-L-alanine Amidase from Bacillus subtilis Strain 168Microbiology, 1984