A Convenient High Yield Synthesis of N4-Isobutyryl-2′-O-Methylcytidine and Its Monomer Units For Incorporation into Oligonucleotides
- 1 December 1993
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 12 (10) , 1007-1013
- https://doi.org/10.1080/07328319308016200
Abstract
We designed an efficient three step procedure for the synthesis of N4-isobutyryl-2′-O-methylcytidine. This protected nucleoside was then used to prepare a methylphosphonamidite monomer for incorporation into oligonucleotides. Transamination at the C4 position of cytidine using ethylenediamine, which has been reported for the N4-benzoyl cytidine, was not observed with N4-isobutyryl protected 2′-O-methylcytidine.Keywords
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