PRODUCTION OF SPORES BY A PUTREFACTIVE ANAEROBE,

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Abstract
An aparticulate medium and a technique were developed for the rapid production of spores of putrefactive anaerobe 3679. The sporulation medium contains trypticase, 1.5%; peptone, 1%; glucose, 0.2%; NaCl, 0.5%; and dipotassium phosphate, 1.25%. Activation of the cultures and stirring of the medium made possible high yields of spores in 44 hours. After sporulation, the cultures were aerated briefly, freeing spores from their sporangia and causing lysis of the remaining vegetative cells. The spores were harvested and washed by centrifugation and could be stored at 4[degree]C in water or phosphate buffer without germinating.
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