ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation
- 1 May 2000
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 405 (6783) , 195-200
- https://doi.org/10.1038/35012097
Abstract
Transcription factors carry functional domains, which are often physically distinct, for sequence-specific DNA binding, transcriptional activation and regulatory functions. The transcription factor ATF-2 is a DNA-binding protein that binds to cyclic AMP-response elements (CREs), forms a homodimer or heterodimer with c-Jun, and stimulates CRE-dependent transcription1,2,3. Here we report that ATF-2 is a histone acetyltransferase (HAT), which specifically acetylates histones H2B and H4 in vitro. Motif A, which is located in the HAT domain, is responsible for the stimulation of CRE-dependent transcription; moreover, in response to ultraviolet irradiation, phosphorylation of ATF-2 is accompanied by enhanced HAT activity of ATF-2 and CRE-dependent transcription. These results indicate that phosphorylation of ATF-2 controls its intrinsic HAT activity and its action on CRE-dependent transcription. ATF-2 may represent a new class of sequence-specific factors, which are able to activate transcription by direct effects on chromatin components.Keywords
This publication has 31 references indexed in Scilit:
- The many HATs of transcription coactivatorsTrends in Biochemical Sciences, 2000
- Structure of the Histone Acetyltransferase Hat1Cell, 1998
- Transcriptional activation by recruitmentNature, 1997
- The CBP co-activator is a histone acetyltransferaseNature, 1996
- The Transcriptional Coactivators p300 and CBP Are Histone AcetyltransferasesPublished by Elsevier ,1996
- A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1ANature, 1996
- Tetrahymena Histone Acetyltransferase A: A Homolog to Yeast Gcn5p Linking Histone Acetylation to Gene ActivationCell, 1996
- Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.Genes & Development, 1994
- Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.Genes & Development, 1989
- Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin geneNature, 1987