Cloning and primary structure of the chiA gene from Aeromonas caviae
- 1 July 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (14) , 4187-4189
- https://doi.org/10.1128/jb.177.14.4187-4189.1995
Abstract
The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.Keywords
This publication has 12 references indexed in Scilit:
- Crystal structure of a bacterial chitinase at 2.3 Å resolutionStructure, 1994
- The chitinase encoding Tn7-based chiA gene endows Pseudomonas fluorescens with the capacity to control plant pathogens in soilGene, 1994
- Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity.Journal of Biological Chemistry, 1993
- Cloning, sequence, and expression of a chitinase gene from a marine bacterium, Altermonas sp. strain O-7Journal of Bacteriology, 1993
- Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinasesJournal of Bacteriology, 1992
- Cloned chitinases in fungal plant-pathogen control strategiesTrends in Biotechnology, 1992
- Evidence that chitinase produced by Aeromonas caviae is involved in the biological control of soil-borne plant pathogens by this bacteriumSoil Biology and Biochemistry, 1991
- Control of Plant Diseases by Chitinase Expressed from Cloned DNA inEscherichia coliPhytopathology®, 1989
- Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homologyJournal of Bacteriology, 1986
- A bacteriophage lambda vector for cloning with BamHI and Sau3AGene, 1982