The absorption spectrum of vitamin A

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Abstract
The absorption spectra of numerous oils and concentrates were examined in an attempt to sustain the assumption that selective absorption by such substances in the region 320-328 [mu][mu] is connected with vitamin A activity. In general there was good agreement between the SbCl3 test and the definition of the absorption band in this region. Aeration or irradiation under conditions known to destroy vitamin A caused a simultaneous failure of the color reaction and disappearance of the 320-328 [mu][mu] absorption band. A dogfish-liver oil known to be rich in vitamin A failed to show a well defined 320-328 [mu][mu] band; this discrepancy is explained as a masking of this band by a relatively high selective absorption in the region of 280 [mu][mu]. The idea is advanced that a decomposition product of vitamin A is characterized by an absorption band at 275-285 [mu][mu].

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