A Stability-Indicating Liquid Chromatographic Method for the Analysis of Erythromycin in Stored Biological Fluids Using Amperometric Detection

Publisher Website
Google Scholar
Abstract
A simple, sensitive and reliable high-performance liquid chromatographic procedure has been developed for the determination of erythromycin in human serum and urine using amperometric detection. A solid-phase extraction procedure was used followed by chromatography on a reverse-phase column. The mean recovery of erythromycin from serum and urine was 80%. Calibration plots for erythromycin base in serum and urine were linear over the ranges 0.25–5.0 μg/ml and 1.25–25.0 μg/ml respectively, with a sensitivity limit of 0.1 μg/ml. This method allows both erythromycin and its principle degradation product, anhydroerythromycin, to be determined during a period of sample storage at 4°C and −15°C. The method is sufficiently sensitive and precise and is thus highly suited for use in both pharmacokinetic and stability studies.