BASIC AND SPECIAL STAINS FOR PLASTIC SECTIONS IN BONE-MARROW HISTOPATHOLOGY, WITH SPECIAL REFERENCE TO MAY-GRUNWALD GIEMSA AND ENZYME-HISTOCHEMISTRY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 28  (3) , 265-279
Abstract
A battery of morphological, histochemical, and enzyme histochemical stains were experimented on semithin sections of glycol-methacrylate-embedded [humans] bone marrow biopsies. It was possible to reproduce on sections the typical ''Romanowsky effect'' which caracterizes May-Gruenwald Giemsa-stained smears of bone marrow or peripheral blood. This appears to be of critical importance for proper routine morphological evaluation of bone marrow biopsies. Conventional histochemical stains, and the enzyme histochemistry reactions that are most useful and widely used in the study of marrow aspiration smears were successfully applied to plastic sections: in this way the evaluation of the cytochemical profiles of marrow diseases, especially leukemias, may be included in the histopathologist''s diagnostic approach, with the additional advantage of preserving the architecture of the tissue and the relationship between hemopoietic cells and stromal components.

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