Immunological cross-reactivity of the fragments of staphylococcal enterotoxins A and E generated by digestion of proteolytic enzymes.

1 January 1991

journal article
Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science

Vol. 53 (2) , 223-227
https://doi.org/10.1292/jvms.53.223

Abstract

Three major fragments were generated by limited digestion of staphylococcal enterotoxins A (SEA) and E (SEE) with papain, whereas five major fragments were generated by limited digestion with staphylococcal protease V8 (V8). All of these fragments were detected by immunoblotting with polyclonal anti-SEA and -SEE sera. Some of generated fragments were detected by monoclonal antibodies (MAbs) with specificities for SEA (A-111 and A-211), SEE (E-142), or both (AE-32, AE-37, and AE-53). This indicates that fragments of SEA and SEE containing the type-specific and cross-reacting epitopes may be generated by digestion of the toxins with either papain or V8.

Keywords

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