A REACTION OF BACTERIAL CELLS WITH FORMALDEHYDE
- 1 May 1950
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 59 (5) , 617-623
- https://doi.org/10.1128/jb.59.5.617-623.1950
Abstract
Treatment of cells of Gram-positive bacteria with neutral HCHO destroys the ability of certain of the cells to react with basic dyes. The relative proportion of cells so reacting is a function of the age of the culture. Old cultures consist predominantly of cells that fail to stain after treatment. Cultures in the logarithmic phase of growth often contain approx. equal numbers of stainable and non-stainable cells after treatment. The effect is prevented or reversed by treating the cells with H ions, alcohol, picric acid, osmic acid, surface-active agents, or even brief air drying.Keywords
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