Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes
- 14 June 2006
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 12 (8) , 1514-1520
- https://doi.org/10.1261/rna.101606
Abstract
In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for loading siRNA onto the siRISC complex. Consistent with this, only the Dcr-2/R2D2 complex, but neither Dcr-2 nor R2D2 alone, can efficiently interact with duplex siRNA. Furthermore, both dsRNA-binding domains of R2D2 are critical for binding to siRNA and promoting assembly of the siRISC complexes.Keywords
This publication has 16 references indexed in Scilit:
- Passenger-Strand Cleavage Facilitates Assembly of siRNA into Ago2-Containing RNAi Enzyme ComplexesPublished by Elsevier ,2005
- Processing of Pre-microRNAs by the Dicer-1–Loquacious Complex in Drosophila CellsPLoS Biology, 2005
- Perspective: machines for RNAiGenes & Development, 2005
- A Protein Sensor for siRNA AsymmetryScience, 2004
- A Dicer-2-Dependent 80S Complex Cleaves Targeted mRNAs during RNAi in DrosophilaCell, 2004
- Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing PathwaysCell, 2004
- R2D2, a Bridge Between the Initiation and Effector Steps of the Drosophila RNAi PathwayScience, 2003
- RNA interferenceNature, 2002
- The dsRNA Binding Protein RDE-4 Interacts with RDE-1, DCR-1, and a DExH-Box Helicase to Direct RNAi in C. elegansCell, 2002
- Role for a bidentate ribonuclease in the initiation step of RNA interferenceNature, 2001